Myelotracker+ [Cy3], Ref MT007. This MyeloTracker is proteolysis-resistant, potentially for investigation with living cells or biologically-active cell extract.
Myelotracker+ [Cy3] is a proteolysis-resistant synthetic conjugated peptide supplied at a 1 mg/mL concentration. Sample purifity has been validated by HPLC. It is recommended to store aliquots at -20°C upon reception.
Myelotracker+ [Cy3] may be used to stain living cell degranulation or for intravital imaging.
Flow cytometry or fluorescent imaging. It is recommended to label cells or tissues at a 1 microg/mL final concentration in PBS with permeabilizer (0.1% saponin or Triton) for myeloid cell intracellular/extracellular staining or without permeabilizer for labelling cell degranulation components.