Myelotracker [Cy5], Ref. MT002.
Myelotracker [Cy5] is a synthetic conjugated peptide supplied at a 1 mg/mL concentration. Sample purifity has been validated by HPLC. It is recommended to store aliquots at -20°C upon reception.
Myelotracker [Cy5] should be used only on fixed samples. For labeling living cells or intravital imaging, Myelotracker+ conjugated markers are recommended.
Flow cytometry or fluorescent imaging. It is recommended to label cells or tissues at a 1 microg/mL final concentration in PBS with permeabilizer (0.1% saponin or Triton) for myeloid cell intracellular/extracellular staining or without permeabilizer for labelling cell degranulation components.